Education


2010 - 2015

Ph.D. Molecular Genetics - University of Tarbiat Modares

Thesis: Design and Production of Chimeric Protein Containing Omp19, Omp31 and Urease against Brucella and Evaluation of its Immunogenicity


2007 - 2010

MSC Cellular and Molecular Biology - Common Student Shahid Madani University of Azarbaijan & National Institute of Genetic Engineering

Thesis: Functional Analysis of Mutated Ribosomal Protein L3 (RPL3 W258C/H259Y) in Yeast towards Conferring Resistance to Mycotoxin Deoxynivalenol


2002 - 2006

BSC Cellular and Molecular Biology Genetics - University of Esfahan

Professional Experiences


2020 - now

Assistant Professor in Genetics Department - Department of Genetics, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran. P.O. Box: 8179849999. Tel: +98-31-36502820. Fax: +98-31-36502825


June 2017 - June 2018

Postdoc researcher - Shiraz University of medical sciences

Thesis: Production of chitosan based nanovaccine containing Brucella melitensis Omp31 and investigation of its immunogenicity in mouse model

Research Interests

  • Detection of Different Pathogens by Molecular Methods
  • Production of Recombinant Proteins in Different Host Cells
  • Vaccine Design by Bioinformatics Tools
  • Function Evaluation of Different Vaccine Delivery Systems

Publications

Design of a chimeric DNA Vaccine against Brucella spp. Minerva - Biotecnologica 2014; 26:223-233

Morteza Abkar

Abbas Sahebghadam Lotfi

Jafar Amani

Seyed Ali Ghorashi

Gholamreza Nikbakht Brujeni

Mehdi Kamali

Subcutaneous immunization with a novel immunogenic candidate (urease) confers protection against Brucella abortus and Brucella melitensis infections. - APMIS 2015 Aug; 123(8): 667-675

Morteza Abkar

Jafar Amani

Abbas Sahebghadam Lotfi

Gholamreza Nikbakht Brujeni

Saeed Alamian

Mehdi Kamali

Survey of Omp19 immunogenicity against Brucella abortus and Brucella melitensis: Influence of nanoparticulation and route of administration versus traditional immunization - Vet Res Comm. 2015 Dec; 39(4):217-28

Morteza Abkar

Abbas Sahebghadam Lotfi

Jafar Amani

Khadijeh Eskandari

Mehdi Fasihi Ramandi

Jafar Salimian

Gholamreza Nikbakht Brujeni

Saeed Alamian

Mehdi Kamali

Hamid Koushki

Mitochondrial Complex I Gene Variations; as a Potential Genetic Risk Factor in Pathogenesis of Multiple Sclerosis - J Neurol Sci 345. 2014. 220-223

Ahmad Poursadegh Zonouzi

Saeid Ghorbian

Morteza Abkar

Ali Akbar Poursadegh Zonouzi

Ali Azadi

Methodology to Understand How Perforin Assembles on Membranes - Scandinavian journal of immunology. 80. 2014. 73-74

Saeid Ghorbian

Morteza Abkar

Ahmad Poursadegh Zonouzi

Asses ability of mutant variety of ribosomal protein L3 (RPL3 W258C/H259Y) in resistance to mycotoxin of Fusarium graminearum by using Saccharomyces cervisiae - Crop Biotech. 2013. Persian. 13-23

Morteza Abkar

F. Sanjarian

M. Mousavi

Recombinant plasmid KMP-11 gene Leishmania major (pcKMP-11): production, characterization and sequencing - Minerva Biotecnologica 2014; September; 26 (3):175-182

Bandani E

Soflaei S

Khalili F

Aghei Afshar M. A

Kooshki H

Abdoli A

Kamali M

Sarvi S

Nasiri V

Jafari A. A

Abkar M

Recent Applications of DNA Vaccines in Cancer Therapy - Molecular medicine journal. 2016. Vol 1, Num 3

Ali Akbar Poursadegh Zonouzi

Ahmad Poursadegh Zonouzi

Morteza Abkar

Optimization of a method for refolding of bacterial recombinant proteins - Molecular and biochemical diagnosis journal. Vol.2, No.1 (2016), 65-78.

Morteza Abkar

Abbas Sahebghadam Lotfi

Jafar Amani

Mehdi Fasihi Ramandi

Using gold nanoparticles in diagnosis and treatment of melanoma cancer - Artificial Cells, Nanomedicine, and Biotechnology, 2018.

Salman Bagheri

Maryam Yasemi

Elmira Safaie-Qamsari

Jamal Rashidiani

Morteza Abkar

Mahmoud Hassani

Seyed Ali Mirhosseini

Hamid Kooshki

Oral immunization of mice with Omp31 loaded N-trimethyl Chitosan nanoparticles induces high protection against B. melitensis infection - International journal of nanomedicine. 2017:12 8769–8778.

Morteza Abkar

Mahdi Fasihi Ramandi

Hamid Kooshki

Abbas Sahebghadam Lotfi

Intraperitoneal immunization with Urease loaded N-trimethyl Chitosan nanoparticles elicits high protection against B. melitensis and B. abortus infections - Immunology Letters. 199 (2018) 53–60.

Morteza Abkar

Mahdi Fasihi Ramandi

Hamid Kooshki

Abbas Sahebghadam Lotfi

CdS nanocrystals/graphene oxide-AuNPs based electrochemiluminescence immunosensor in sensitive quantification of a cancer biomarker: p53 - Biosensors and Bioelectronics. Volume 126, 1 February 2019, Pages 7-14.

Reza Heidari

Jamal Rashidiani

Morteza Abkar

The neuroprotective effect of BSA-based nanocurcumin against 6-OHDA-induced cell death in SH-SY5Y cells - Avicenna Journal of Phytomedicine. 2018. In press

Roksana Sookhaklari

Bita Geramizadeh

Morteza Abkar

Maryam Moosavi

Curcumin Loaded BSA Nanoparticles Protects More Efficiently Than Natural Curcumin Against Scopolamine￾Induced Memory Retrieval Deficit - Basic and Clinical Neuroscience. 2018.

Maryam Moosavi

Roksana SoukhakLari

Leila Moezi

Fatema Pirsalami

Morteza Abkar

Association of hsa-miR5571-5p expression with clinicopathological factors and identification of its hub target genes and key pathway in breast cancer - Iranian Journal of Public Health. 2022. Under print.

Mahshid Samie Ghahfarokhi

Somayeh Reiisi

Zahra Zamanzadeh

Morteza Abkar

Talks

Brucella abortus Outer Membrane Protein 19 Loaded N-trimethyl Chitosan Confers Protection against B. abortus, B. melitensis and B. suis in Mice - 12th international congress of immunology & allergy. Lecture. 2014. Tehran. Iran

Abkar M

Sahebghadam Lotfi A

Amani J

Eskandari K

Fasihi M

Nikbakht Brujeni G

Alamian S

Kamali M

The Oral Chimeric Vaccine Candidate Omp19-Omp31 Induces Protection against Different Brucella spp Challenges by Inducing IL-17 Immune Response - 12th international congress of immunology & allergy. Lecture. 2014. Tehran. Iran.

Abkar M

SahebghadamLotfi A

Amani J

Eskandari K

Fasihi M

Nikbakht Brujeni G

Kamali M

Koushki H

Influence of Administration Route on Omp31 immunogenicity against Brucella melitensis - 13th international congress of immunology & allergy. Lecture. 2016. Tabriz. Iran

Abkar M

Poursadegh Zonouzi A

Fasihi Ramandi M

Sahebghadam Lotfi A

Examination of Urease immunogenicity against Brucella abortus and Brucella melitensis: influence of nanoparticulation versus traditional immunization - 13th international congress of immunology & allergy. Lecture. 2016. Tabriz. Iran.

Abkar M

Nikbakht Brujeni G

Poursadegh Zonouzi A

Fasihi Ramandi M

Sahebghadam Lotfi A

Gelatin nanoparticle based delivery of Urease and Omp31 in mice protects against Brucella melitensis 16M infection14th international congress of immunology & - allergy. Lecture. 2018. Tehran. Iran.

Abkar M

Alamian S

Sattarahmady N

Subcutaneous vaccination with aluminium hydroxide/Omp31 and chitosan/Omp31 nanoparticles induces protection against Brucella melitensis infection in BALB/c mice - 14th international congress of immunology & allergy. Lecture. 2018. Tehran. Iran.

Abkar M

Alamian S

Sattarahmady N

Oral Administration of Mice with Chimeric Protein Containing Urease, Omp31 and Omp19 Induces High Protection against Brucella melitensis and Brucella abortus Infections - 14th international congress of immunology & allergy. Lecture. 2018. Tehran. Iran.

Abkar M

Alamian S

Fasihi Ramandi M

Sahebghadam Lotfi A

Posters

In silico analysis of yeast acetyl transferase (AYT1) - The 4th Iranian conference on Bioinformatics. Poster. 2012. Tehran

Abkar M

Sanjarian F

Cloning, expression, purification and in silico analysis of the Brucella Urease - 3rd international student biotechnology congress. Poster. 2013. Tehran. Iran.

Abkar M

Sahebghadam Lotfi A

Amani J

Association between DROSHA rs642321 Polymorphism and Breast Cancer in Iranian Women. International conference on human genetics and genomics. Poster - Yazd. December 2021.

Taghipour Kamalabad S

Zamanzadeh Z

Rezaei H

Tabatabaeian M

Abkar M

rs10719T> polymorphism in DROSHA gene may destroy binding site of has-miR-664a-3p and has-miR-1298-5p. - International conference on human genetics and genomics. Poster. Yazd. December 2021.

Taghipour Kamalabad S

Zamanzadeh Z

Rezaei H

Tabatabaeian M

Abkar M

In silico polyepitope vaccine design against SARS-COV-2 infection - International conference on human genetics and genomics. Poster. Yazd. December 2021.

Owji F

Jamali S

Akbar M

Design of a Common Vaccine against Influenza and SARS-COV-2 - Viruses by Using In Silico Tools. International conference on human genetics and genomics. Poster. Yazd. December 2021.

Jamali S

Owji F

Abkar M

Co-infection of Gardnella vaginalis and human papillomavirus in cervical samples: A multicenter study in Iran. Iran 22nd - international virtual congress of microbiology. Poster. Tehran. September 2021.

Shelerangkon M

Khodabakhsh M

Moein B

Nasri E

Abkar M

Fakhim H

characterization of airway yeast colonization in tracheal aspirates of patients with SARS-CoV2 infection admitted to the intensive care unit in Isfahan, Iran - Poster. Tehran. September 2021

Khodabakhsh M

Shelerangkon M

Moein B

Nasri E

Abkar M

Fakhim H. Molecular

Patents

  • Iran Patent Center - patent No: 87917
  • Iran Patent Center - patent No: 99547

Teaching Experience

University Courses

  • Basic Genetics
  • Molecular Genetics
  • Immunology
  • Topics in Genetics
  • Genetic Engineering

Workshops

  • Bioinformatics
  • Genome and Protein Database Introducing and Primer Design
  • Real-time PCR
  • Gene Cloning and Bacterial Transformation
  • PCR and Gel Electrophoresis
  • RNA Isolation
  • RT-PCR
  • Principles of DNA Isolation from plants, Animals and Prokaryotic Cells
  • Vaccine Design

Skills

  • Design of blood DNA extraction kit
  • Primer design
  • PCR Types such as RT-PCR, Real-time PCR, Soeing PCR, PCR-RFLP, …
  • Gene cloning, SDS-PAGE & Western Blotting
  • Design and Production of DNA extraction kits from blood, saliva, …
  • Vaccine design
  • ELISA
  • MTT assay

Reference

Prof. Abbas Sahebghadam Lotfi; Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, P.O. Box: 14155-6343, Tehran, Iran. [email protected] .